In vitro propagation of endangered relic plant species Malosorbus florentina
2006
Savic, J.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia) | Devic, M.,Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Plant Physiology | Giba, Z.,University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany | Grubisic, D.,University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany
Malosorbus florentina (Maloideaea, Rosaceae) is a relic plant species distributed in Apennines, Balkans and in Asia Minor. In Serbia this species has been protected by law as a natural rarity. An extremely small population of this species with only several trees was found on Mt. Sokolovica in central part of Serbia. Fruits with seeds were collected from these plants in 2003. In order to save this endangered species we try to develop a protocol for in vitro propagation as one of the ex situ conservation method. In vitro culture was successfully established from seeds after stratification (4 months at + 4 deg C). The best multiplication rate of shoots was obtained on MS medium supplemented with BAB, IBA and GA3, all in concentration of 1 microM. Shoots did not spontaneously form roots in vitro on hormone-free half-strength MS medium. Experiments in order to find optimal conditions for rooting are in progress.
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