In planta transformation of buckwheat (Fagopyrum esculentum Moench.)
2007
Bratic, A.M.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Majic, D.B.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Miljus-Djukic, J.D.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Jovanovic, Z.S.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia) | Maksimovic, V.R.,Institute of Molecular Genetics and Genetic Engineering, Belgrade (Serbia)
In order to develop a reliable and rapid transgenic system for functional study of specific buckwheat gene constructs, two different in planta transformation methods were analyzed: vacuum infiltration and infiltration by syringe. The results indicated that the vacuum infiltration method was much more efficient and can therefore be considered the method of choice for buckwheat transformation.
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