The determination of hyperoside in the flower and leaf of hawthorn (Crataegus oxycantha and Crataegus monogyna) by the spectrophotometric method
2006
Mihajlovic, D., FHI Zdravlje Actavis Company, Leskovac (Serbia). Centar za istrazivanje i razvoj | Novkovic, V., FHI Zdravlje Actavis Company, Leskovac (Serbia). Centar za istrazivanje i razvoj | Bankovic, V., FHI Zdravlje Actavis Company, Leskovac (Serbia). Centar za istrazivanje i razvoj
The extraction of the flavonoids from a mixture of howthorn (C. oxycantha and C. monogyna) flower and leaf was carried out by percolation with 40% and 70% (v/v) ethanol. In order to determine the content of total flavonoids calculated as hyperoside, spectrophotometric method with aluminium (III) chloride as a colour reagent at 425 nm was applied. The total flavonoid content in the herbal material mixture was 0.483%, in 40% and 70% (v/v) ethanolic liquid extract it was 138 mg/100 cubic cm and 157 mg/100 cubic cm, respectively. The extraction degree of total flavonoids with 40% and 70% (v/v) ethanolic solutions was 57.14% and 65.01%, respectively. The yield of total flavonoids in the dry extract was 63% in regard to the herbal mixture content.
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