Investigation of biofilm formation in vitro ability of Listeria monocytogenes strains isolated from animals
2007
Milanov, D., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Asanin, R., Faculty of Veterinary Medicine, Belgrade (Serbia) | Misic, D., Faculty of Veterinary Medicine, Belgrade (Serbia) | Vidic, B., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Ratajac, R., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)
In this paper we presented the results obtained for four strains of Listeria monocytogenes isolated from brain samples of sheep, designed as 785/05, 593/05, 748/05, 1915/04 and one strain isolated from aborted calves fetus, designed as 021/04. The referent strain of L. monocytogenes, 1071 (4b), was used as a control. Strains of L. monocytogenes cultured in nutrient-rich media (e.g. TSB-YE) and at higher temperatures (37 deg C) exhibited a higher ability of biofilm formation. However, non of the studied strains could be classified as a good biofilm producer, disregard of the incubation temperature and medium used. The highest OD values were obtained at the incubation tempareture of 37 deg C in TSB-YE (OD595=0.346+/-0.055), when three strains were quantified as moderate and two as weak biofilm producers. The decrease of incubation temperature resulted in decreased OD values, thus four strains were classified as weak biofilm producers at 25 deg C (OD595=0.289+/-0.083), and none of the investigated strains was assessed as biofilm producers at 4 deg C (OD595=0.124+/-0.011). Light-microscopy examination, confirmed by quantitative values obtained by the crystal violet microtiter test, proved to be a simple and rapid screening method for the quantification of the ability of L. monocytogenes to form biofilms in varying test conditions.
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