Evaluation of genotoxic effects of fumagillin by sister chromatid exchange and chromosomal aberration tests in human cell cultures
2007
Stanimirovic, Z., Faculty of Veterinary Medicine, Belgrade (Serbia) | Pejin, I.I., Faculty of Veterinary Medicine, Belgrade (Serbia) | Kulisic, Z., Faculty of Veterinary Medicine, Belgrade (Serbia) | Djiporovic, M., Faculty of Forestry, Belgrade (Serbia)
Fumagilllin is a naturally secreted antibiotic of the fungus Aspergillus fumigatus. In veterinary medicine fumagillin is used against nosemosis in bees and microsporidiosis in fish. Since fumagillin is stable in honey in a honey bee hive there is possible genotoxic risk to beekeepers and consumers of honey contaminated with this substance. The genotoxic effect of fumagillin was evaluated in sister-chromatid exchange (SCE) and chromosome aberration tests in cultured human peripheral blood lymphocytes at three concentrations. The results revealed that all tested concentrations of fumagillin (1.02, 3.07 and 9.20 microgram/mL) significantly (P is less than 0.001) increased SCE frequency per cell and decreased the proliferative activity of human cultured lymphocytes which was manifested in the decrease in mitotic and proliferative indices. Significant increase (P is less than 0.001) in aneuploidy and polyploidy was induced by medium and maximum concentrations of fumagillin. Besides, maxumum concentrations of fumagillin significantly (P is less than 0.001) increased the frequency of monitored structural aberrations of gap, break and insertion type. The results of this study demonstrate the genotoxic potential of fumagillin in vitro.
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