Data on the isolation of immunoglobulin from the serum of the green frog (Rana esculenta)
2006
Stosic, S., Clinical Center of Serbia, Belgrade (Serbia). Institute of Otorhinolaryngology and Maxillofacial Surgery | Divjak, I., Clinical Center of Serbia, Belgrade (Serbia). Institute of Otorhinolaryngology and Maxillofacial Surgery | Jesic, S., Clinical Center of Serbia, Belgrade (Serbia). Institute of Otorhinolaryngology and Maxillofacial Surgery | Nesic, V., Clinical Center of Serbia, Belgrade (Serbia). Institute of Otorhinolaryngology and Maxillofacial Surgery | Racic, A., Clinical Center of Serbia, Belgrade (Serbia). Institute of Otorhinolaryngology and Maxillofacial Surgery | Popovic, N., Institute for Medical Research, Belgrade (Serbia)
In this study, an attempt was made to isolate some frog serum proteins and to investigate the electrophoretic qualities of the isolated components. Ig G was isolated using the same procedure applied for human sera and one of the components found in the beta globulin zone was isolated from frog serum by the same procedure. Immunoelectrophoretic analysis, carried out with the full antiserum of a rabbit, obtained by the immunization of the rabbit with the frog serum, showed that the isolated component was pure. The obtained results confirm the fact that there are no slow gamma globulins in the frog serum and point out that this procedure, used for isolating human Ig G class, is also suitable for isolating one protein component from the serum of the frog, which has the electrophoretic speed of beta globulin and which may represent one category of frog immunoglobulins.
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