Elaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds | Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja
2008
Balaz, J., Poljoprivredni fakultet, Novi Sad (Serbia) | Popovic, T., Poljoprivredni fakultet, Novi Sad (Serbia) | Vasic, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Nikolic, Z., Nacionalna laboratorija za ispitivanje semena, Novi Sad (Serbia)
Pseudomonas savastanoi pv. phaseolicola detected on artificially inoculated bean seeds was investigated. The method of the International Seed Federation - ISF (2006) was used. It includes bacteria extraction from seeds, isolation on semiselective media and checking the pathogenicity of investigated isolates. For verification of results, quick new methods of investigation were used (ELISA test and PCR). The results show that semiselective media MT (Milk Tween Agar) and MSP (Modified Sucrose Peptone Agar) can be appropriate for isolation of this bacterium. Pathogenicity of the investigated isolates was confirmed on cotyledon leaves of bean. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium P.s.pv.phaseolicola.
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