Extraction and partial purification of coagulation active components from common bean seed
2006
Sciban, M.B., University of Novi Sad, Novi Sad (Serbia). Faculty of Technology | Antov, M.G., University of Novi Sad, Novi Sad (Serbia). Faculty of Technology | Klasnja, M.T., University of Novi Sad, Novi Sad (Serbia). Faculty of Technology
An active coagulation active component was extracted from common bean seed by NaCl solution and the obtained crude extract was partially purified through a sequence of steps that included precipitation of protein by ammonium sulphate, desalting by dialysis and anion exchange. A turbid water was treated by protein fractions obtained in the anion-exchange elution process by stepwise increase in NaCl concentration. The jar tests were conducted at various dosages of eluates. Different mode of relation between coagulation activity and applied coagulant dose for each protein fraction indicated the existence of different mechanisms of coagulation/flocculation, depending on characteristics of different proteins in the fractions.
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