Identification of hydrolytic and oxydoreductase enzymes in the primary leaves extracts of cereals infected with powdery mildew
2005
Hlinkova, E.(Univerzita Komenskeho, Bratislava (Slovak Republic). Prirodovedecka fakulta | Perecko, D.Slovenska akademia vied, Bratislava (Slovak Republic). Ustav molekularnej biologie | Bobak, M.Univerzita Komenskeho, Bratislava (Slovak Republic). Prirodovedecka fakulta | Rafay, J.Vyskumny ustav zivocisnej vyroby, Nitra (Slovak Republic) | Kutarnova, Z.Univerzita Komenskeho, Bratislava (Slovak Republic). Prirodovedecka fakulta | Kovacova, Z. | Blehova, A.
In the last period a large attention is focused to the PR and DR-proteins. De novo synthesized PR and DR-proteins are the result of pathogen infection and defense reactions of the host plants. Their quantitative and qualitative content changes in the dependence of the early and late genes expression participating in the defense reactions. Basic proteins patterns contained de novo synthesized PR-proteins with low molecular mass (Mr~13-25 kDa) are localized preferentially in the site of infected leaf of host plants. Basic PR-proteins have the same molecular mass and they were idependent on both host and pathogen genotype. Their biochemical function was from the group of RNA binding proteins and proteins with RN-ase activity. Protein with chitinase activity (Mr~14.4 kda) identified with calcofluor white M2R was specific and its content depended on the genetic background of host-pathogen interaction. Constitutively synthesized basic exo beta-1,3-glucanases had their molecular masses from interval Mr~72-40 kDa. Infection level affected amount of storage proteins by sensitive barley genotype Dvoran preferentially at the albumin-globulin fraction. Contain of hordein-glutenin fraction by compatible interaction was effected slower.
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