Using microsatellite markers for sensitivity testing to FHB in spring barley
2005
Jeziskova, I.(Mendelova zemedelska a lesnicka univ., Brno (Czech Republic) | Wegscheiderova, V. | Bednar, J.
Fusarium Head Blight (FHB) is a fungus disease of cereals widely spread all over the world and is caused by members of the genus Fusarium (F. culmorum, F. graminearum, etc.). FHB infection of barley may result in lower yields and in the accumulation of dangerous secondary metabolites of fungi - mycotoxins in the kernels (e.g. deoxynivalenol, DON). The accumulated mycotoxins have a negative effect on the safety and technological quality of the grain. The objective of the study was to test 7 parental genotypes of spring barley (with a declared resistance and susceptibility to FHB with SSR markers (Simple Sequence Repeats). We tested the following parental genotypes: Chevron, Zao Zhou 3, PEC 210, CI 4196 a 6NDRFG-1 (resistant genotypes), and PI 383933 and Foster (susceptible genotypes). The results of analyses will be used to test 22 DH lines, which arose by crossing the tested parental genotypes (resistant x susceptible). By now a total of 35 SSR markers were tested out of total number of 60 selected microsatellite markers; 29 of the tested SSR markers showed a polymorphous character in the parental genotypes. The remaining 6 tested SSR markers were monomorphous and will not be used to test the DH lines. Partial results of the analyses were elaborated into a binary matrix and transform into the form of a dendrogram, which characterised the genetic affinity of the tested genotypes.
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