Interline diversity analysis in Japanese quail population using V7 RAPD markers
2007
Židek, R., Slovak University of Agriculture in Nitra (Slovak Republic) | Rybanská, M., Slovak University of Agriculture in Nitra (Slovak Republic)
Japanese quail (Coturnix Japonica) has been used as an animal for laboratory research because of its small body size, short generation interval and high egg production. Randomly amplified polymorphic DNA (RAPD) is a powerful tool for studying the genetic similarity between lines or populations. NCH line selected for low cholesterol content, VCH line selected for high cholesterol content and non-selected KCH line (control) were used for this experiment. V7 decamer primer successfully amplified the genomic DNA from samples of Japanese quail lines. A total of 9 fragments were observed by individual analysis, 8 bands were polymorphic. Increase in the frequency of polymorphic bands was detected in all the selected lines. Computed genetic similarity ranged from 0.836 to 0.983. Lowest value of genetic similarity was observed between the line with high cholesterol content and the line with low-cholesterol content. Random markers are able to detect genetic similarities between analysed lines of animals, and predict genetic marker searching tool in Japanese quail population.
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