Influence of different non starch polysaccaride degrading feed enzymes on the intestinal microbiota in piglets
2006
Osswald, T.,Institute of Animal Nutrition, Berlin (Germany) | Vahjen, W.,Institute of Animal Nutrition, Berlin (Germany) | Simon, O.,Institute of Animal Nutrition, Berlin (Germany)
In order to estimate the growth potential of intestinal bacteria under the influence of different types of enzymes, 1,4 alpha-arabinoxylan from wheat, 1,3 - 1,4 alpha-glucan from barley or modified wheat extracts were preincubated for 1 hour with an endo 1,4 alpha-xylanase- or amultienzyme preparation before incubation with intestinal contents of weaned piglets (56d). Compared to controls, both enzyme preparations inhibited bacterial growth in stomach contents in incubations with 1,4- alpha arabinoxylan as substrate, but the multienzyme preparation led to higher bacterial growth in jejunum and colon contents. A growth increase was noted for the multienzyme preparation and 1,3 - 1,4- alpha glucan as substrate in all intestinal segments, while the monoenzyme showed no difference with this substrate. A trend for growth reduction for both enzyme preparations in stomach- and in part in jejunum contents was also visible in incubations with a large molecular size wheat extract ( 30 kD). Only minor differences were observed for its low molecular counterpart ( 30 kD) and endo 1,4 alpha-xylanase preincubation, but the presence of the multienzyme preparation showed increased growth. This in vitro study has shown that intestinal bacteria react differently to the presence of non - starch polysaccharide (NSP) degrading enzymes. Production of multiple NSP fragments by the multienzym preparation from the wheat extracts may enhance bacterial growth in the jejunum, while the hydrolysis of 1,4- alpha arabinoxylans with the 1,4- alpha arabinoxylanase seemed to inhibit bacteria in all tested intestinal segments.
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