Generic distinction in the Helminthosporium-complex based on restriction analysis of the nuclear ribosomal RNA gene
1998
Goh, T.K., The University of Hong Kong,Pokfulam Road (Hong Kong). Department of Ecology and Biodiversity | Hyde, K.D., The University of Hong Kong,Pokfulam Road (Hong Kong). Department of Ecology and Biodiversity | Lee, D.K.L., The Hong Kong Polytechnic University, Hung Hom (Hong Kong)
The DNA sequences coding for the nuclear 28S Rrna, 5.8S rRNA, and the internal transcribed spacers (ITSI and ITS2) for 36 representative fungal species from Bipolaris, Cercospora, Corynespora, Curvularia, Drechslera, Exserohilum and Helminthosporium were amplified using the polymerase chain reaction. The amplified products were digested using restriction endonuclease Rsa I, and the electrophorectic patterns compared. With the exception of Bipolaris and Curvularia, cluster analysis of the restriction phenotypes clearly separated the genera. Bipolaris and Curvularia appeared to be more heterogeneous than the other allied genera within the Helminthosporium-complex.
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