Influence of flowcytometry on the quality of bull sperm
2009
Todorov, Ya., Institute of Animal Science, Kostinbrod (Bulgaria) | Todorova, T., Institute of Animal Science, Kostinbrod (Bulgaria)
The objectives of the study were to analyze the effects of semen processing after flow-cytometrical sorting and subsequent freezing in liquid nitrogen on the quality of frozen - thawed spermatozoa. The quality of the frozen - thawed semen was evaluated by motility estimation, morphology analysis and the acrosomal integrity. Prolonged high dilution of flow-cytometrically sorted spermatozoa before freezing had a significant negative effect on the motility and capacitation status. Positive effects on membrane stability were observed when glycerol was added at 5°C shortly before straws were frozen compared to a system where glycerol was added before the equilibration process at room temperature. Independently of sperm processing after sorting, the sexed spermatozoa had significantly more damaged acrosomes and morphological abnormalities. After incubation at 37°C for 3 h and 6 h the motility of the sorted spermatozoa was significantly lower compared to that of the unsorted spermatozoa.
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