Non-surgical transfer of porcine embryos to different uterine sites and effect of transferred embryos number on monitored parameters
2008
Urbankova, L.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic) | Vejnar, J.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic) | Rozkot, M.,Vyzkumny Ustav Zivocisne Vyroby, Prague (Czech Republic)
The first aim of this study was to compare two methods of non-surgical embryo transfer in pigs. In the first method embryos were placed into the uterine body, in the second method embryos were placed approximately into the middle of a uterine horn. Embryos were recovered post mortem from superovulated donors. Recipients were synchronized by feeding Regumate and by applying PMSG and HCG (gilts) or by weaning the piglets (sows). In the first method an insemination probe completed with silicon rigid catheter was used for embryo transfer. Using this probe embryos were put into the corpus uteri. In the second method the intrauterine insemination probe Verona completed with silicon rigid catheter was used. Using this method embryos were put into the middle of one of the uterine horns. Forty-two % and 56% of the recipients remained pregnant, respectively. The litter size was 5.5 vs. 6.6 piglets, embryos survival rate was 10 vs. 11%. The second objective of this study was to evaluate the effect of number of transferred embryos on the number of born piglets. Recipients were divided into 3 groups according to the amount of transferred embryos. From 5 to 15 embryos were transferred into recipients in the first group (A), from 16 to 25 embryos in the second group (B), and 26 and more in the third group (C). Forty-two, 52 and 50% of the recipients remained pregnant, respectively. Embryos survival rates were 14, 11 and 7%, resp.
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