A novel method for determining total vitamin B1 in processed food enriched with dibenzoyl thiamine
2008
Yoshida, M.(Japan Food Research Labs., Tama, Tokyo. Tama Lab.) | Hishiyama, T. | Igarashi, T.
Dibenzoyl Thiamine (DBT), a lipophilic derivative of vitamin Bsub(1), has been approved as a food additive in Japan. The use of DBT in a variety of processed food has increased recently due to the current consumer trend for 'Vitamin-Rich' food products. However, there are no current methods that accurately measure the total vitamin Bsub(1) in DBT-rich food due to the incomplete hydrolysis of DBT to thiamine. Although Takadiastase generally has been used for thiamine determination to liberate free-thiamine from its esterified forms, this incomplete hydrolysis of DBT causes underestimation of the total vitamin Bsub(1) in processed food. Therefore, we added an alkaline-hydrolysis-step with NaOH to the Japanese official method for the nutritional food labeling to optimize the determination of total vitamin Bsub(1). After dephosphorylation using Takadiastase and complete hydrolysis of DBT to thiamine in 0.49mol/L NaOH, total vitamin Bsub(1) was determined by the post-column HPLC method combined with a column-switching system. Through examining more than 60 samples of processed food with or without DBT, no significant matrix effects were observed. The recovery test obtained a little over 90% recovery and around 2% RSD for more than 20 samples containing DBT. Based on these results, we propose a novel and single validated procedure for accurately measuring the total vitamin Bsub(1) content in processed food regardless of DBT content.
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