Identification of the attached chromosome in sex-limited normal marked silkworm, Bombyx mori
2008
Ikejima, S.(Institute of Sericulture, Ami, Ibaraki (Japan)) | Tsuneyama, I. | Tanaka, Y. | Ninagi, O. | Hara, W.
Genomic Southern blot hybridization of two sex-limited silkworm strains, W3a and W73, which show the female specific larval markers, crescent mark and star spot, were carried out to identify the areas of attached second chromosome by using RFLP probes of the ESTcDNA clones. When the two clones, e61 and m113 on the second linkage group were used, W3a showed female specific bands. On the other hand, even if these clones were used, W73 showed no difference in the band pattern between females and males. When other clones on the second linkage group were used, both strains showed no difference in the band pattern between females and males. This result indicated that the area of e61-m113 was included in the attached chromosome of W3a. The difference of the area of the attached chromosome between these strains suggested that the length of the W3's attached chromosome was longer than W73's.
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