Overproduction of glutathione by L-cysteine addition and a temperature-shift strategy
2008
Wei, Gong-Yuan (Soochow University, Suzhou, China), E-mail: [email protected] | Wang, Da-Hui (Soochow University, Suzhou, China) | Chen, Jian (Jiangnan University, Wuxi, China)
The present study investigated the effects of three constituent amino acids on glutathione production in flask culture of Candida utilis. Although L-glutamic acid and glycine had little impact on cell growth and glutathione biosynthesis, L-cysteine positively influenced glutathione production, despite inhibiting cell growth when it was added prior to stationary phase. Adding 8 mmol/L of L-cysteine to the culture broth at 16 h boosted glutathione production by 91%, increasing the intracellular glutathione content by 106% compared to untreated controls. A temperature-shift strategy, in which we shifted batch and fed-batch cultures of C. utilis from 30 to 26℃, also significantly enhanced glutathione production. Applying both strategies (i.e. adding 20 mmol/L L-cysteine and shifting the temperature from 30 to 26℃) at 33 h enhanced the glutathione concentration and the intracellular glutathione content to 1,312 mg/L and 3.75%, respectively, during fed-batch cultivation (glucose feeding at a constant rate of 18.3 g/h). The average specific glutathione production rate under this condition was 129% higher than that of the control without strategy.
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