Reactivity of German Cockroach Allergen, Bla g 2, Peptide Fragments to IgE Antibodies in Patients' Sera
2008
Lee, H.S. (Yonsei University College of Medicine, Seoul, Republic of Korea) | Jeong, K.Y. (Yonsei University College of Medicine, Seoul, Republic of Korea) | Shin, K.H. (Yonsei University College of Medicine, Seoul, Republic of Korea) | Yi, M.H. (Yonsei University College of Medicine, Seoul, Republic of Korea) | Gantulaga, Darambazar (Health Science University, Ulan Bator, Mongolia) | Hong, C.S. (Yonsei University College of Medicine, Seoul, Republic of Korea) | Yong, T.S. (Yonsei University College of Medicine, Seoul, Republic of Korea), E-mail: [email protected]
Bla g 2 is a cockroach allergen of great importance. This study was conducted to identify IgE-binding epitope(s) of Bla g 2 using the recombinant protein technique. Approximately 50% of tested sera showed IgE reactivity to Pichia-expressed Bla g 2 (PrBla g 2) and E. coli-expressed Bla g 2 (ErBla g 2). Only 5.3% of serum samples showed stronger reactivity to PrBla g 2 than ErBla g 2, indicating that serum was reactive to conformational or carbohydrate epitopes. The full-length and 5 peptide fragments of Bla g 2 were produced in E. coli. All fragments showed IgE-binding activity to the cockroach-allergy patients' sera. Specifically, peptide fragments of amino acid residue 1-75 and 146-225 appeared to be important for IgE-binding. The information about the IgE-binding epitope of Bla g 2 can aid in the diagnosis and treatment for cockroach allergies.
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