Detection of Toxoplasma gondii infection in butchers and buffaloes by polymerase chain reaction (PCR) and latex agglutination test
2005
Ahmed, R.S.
Toxoplasinosis a common parasitic zoonotic infection is usually aymptoinatic in immunocompetent persons although it may be present as lyinphadenopathy, febrility, etc. but it is a life threatening opportunistic infection in congenitally infected patients and in immunocompromised individuals (those with AIDS, malignancy, organ transplantation, etc). Human; being become with T. gondii usually by ingesting oocysts in food and water contaminated with coat feces or by consuming tissue cysts in undercooked meat. The diagnosis of toxoplasmosis is mainly based on serological tests latex agglutination test (LAT). Detection of specific DNA seems to be of clinical value in the patients infected with toxoplasmosis. In this study, latex agglutination test was used for the detection of the antibodies against Toxoplasma gondii and Polymerase Chain Reaction (PCR) based on the amplification of repetitive B1 gene of T. gondii. The study was base on a total of 200 samples involving 50 butchers, 50 buffalo's sera and whole blood, respectively. LAT established an overall infection of T. gondii in buffaloes as 20% and 22%, respectively. The PCR analysis confirmed this T. gondii prevalence in butchers and buffaloes. Latex Agglutination Test proved to be an efficacious method for routine serological screening for antibodies to T. gondii. The sophisticated Polymerase Chain Reaction results in our investigation showed good correlation with the serological data. The results demonstrated that PCR analysis of clinical samples of patients susceptive for toxoplasmosis can be a promising diagnostic method that enables direct detection of parasitic DNA.
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