Presence of deoxynivalenol in cereals and analytical techniques of determination. [Doctoral dissertation] | Prisustvo deoksinivalenola u zitaricama i analiticke tehnike odredjivanja. Doktorska disertacija
2008
Jajic, I.
For getting a more relevant picture of the state of deoxynivalenol contamination of cereals and other crops produced in Serbia, in this doctoral thesis we investigated numerous cereal samples for the presence and concentration of this mycotoxin. Samples of cereals and other feed, collected during the period 2004-2007 from different locations in the country and especially from Vojvodina, were analyzed by high performance liquid chromatography (HPLC), and also by using rapid methods such as immunochemical (ELISA), and rapid and non-destructive methods such as Fourier transform infrared spectroscopy (FTIR). After comparing the obtained results with the relevant data about presence of DON in the countries of our region, and also in EU, we have concluded that the situation on our country is not worse than in the average of mentioned countries. An encouraging fact is that of the total number of analysed samples, only in 2 maize and 1 wheat sample the concentration range of DON was above the maximum level recommended by European Union Commission for these cultures (1.75 and 1.25 microgram/g, respectively). Our investigation concerned mainly the efficiency of clean-up of cereal crude extracts, especially efficiency of immunoaffinity columns and possibility of their multiple use and regeneration. Solvents used for regeneration were water and phosphate buffer solution (PBS), and for eluation were used methanol and water-methanol mixture in variable volume proportions. The efficiency of clean-up depended primarily on a number of applications of DON spiked solution to the same immunoaffinity column. Also, the results obtained show a clear advantage of the ATR over the DR measuremsts.
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