Establishing the resistance of Myzus persicae (Sulzer) by molecular methods
2008
Vucetic, A. | Petrovic-Obradovic, O., University of Belgrade, Belgrade - Zemun (Serbia). Faculty of Agriculture | Margaritopoulos, J. | Skouras, P., University of Thessaly, Volos (Greece). Department of Crop Production and Agricultural Environment, Laboratory of Entomology and Agricultural Zoology
In two years of investigating resistance of the peach-potato aphid Myzus persicae by molecular method, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetylcholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investigations, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance.
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