Research on genetic structure of a cowberry population (Vaccinium vitis-idaea) in the reserve
2008
Glotov, N.V. | Prokopieva, L.V., Marij-Ehl StateTechnical Univ., Joshkar-Ola (Russian Federation) | Semerikov, V.L.
Genetic structure of a cowberry population in the reserve Bolshaya Kokshaga was examined based on the analyses of 15 allozyrae loci, 10 of those being polymorphic. Partial plants (spaced by no less than 10-15 m), were sampled along 3 routes, being 5-15 km distant from each other; the total number made 122 partial plants Three samples demonstrated similar allele frequencies for the corresponding loci, the average allele number per locus made 1,8-1.9; average heterozygosis values were 0.208-0.227; the observed genotype ratios differed from the values expected after the Hardy-Weinberg relation at 1-5% level of significance. Differentiation of the samples was very low (Fst=0,014), yet statistically significant. Probably one should regard these three samples as parts of the same population. Clonal structure of the population was revealed when the partial plants were collected from the plots not bigger than 4 square m (the sample included 259 partial plants); the lower limit of the estimated clone extent for the sampled plants made about 15 m. Complete excavation and tracing of the rootstocks on the plot of 1 square m, with allozyme identification of partial plants on its separated segments, lead to reveal 7 clones. The biggest clone was marked for the rhizome length of 36.8 m and had 108 partial plants
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