Tissue culture and plantlet regeneration of the axillary bud’s leaf on spring cabbages | 春甘蓝腋芽叶片离体培养及植株再生技术研究

Chino. 探索一种能保留春季栽培春甘蓝亲本春性的育种方法。以春季田间栽培的春甘蓝MP01-_11152、GB9266-18136、DT409-05121 3个亲本自交系为试材，选用其成熟叶球腋芽叶片为外植体，首先以MS为基础培养基，添加0，2，3，4，5 mg/L 6-苄基腺嘌呤（6-BA）、0，0.05，0.1，0.2，0.3，0.4，0.5 mg/L α-萘乙酸（NAA）对MP01-11152亲本自交系进行筛选，筛选出6-BA和NAA的最佳质量浓度；然后利用已选出的6-BA和NAA最佳质量浓度，分别对供试3个品种进行腋芽叶片离体培养及植株再生研究。以MP01-11152为材料进行离体培养，筛选出6-BA和NAA的最佳质量浓度分别为2和0.4 mg/L。3个亲本自交系中，GB9266-18136在MS+2 mg/L 6-BA+0.4 mg/L NAA中诱导的不定芽分化率最高，为211%；MP01-11152次之，为122%；DT409-05121最低，为82%。将诱导出的不定芽在 MS+0.3 mg/L NAA生根培养基中诱导生根，不定根的诱导频率可达98%，并锻炼生长成苗。在MS培养基中添加适宜质量浓度的6-BA或NAA 2种激素，能够有效地提高春甘蓝腋芽叶片不定芽的诱导分化率，基因型对不定芽诱导的分化率有一定影响。

Inglés. This passage discusses the method for keeping the springness from the leaf of the axillary buds on spring, to develop and enhance the breeding of spring cabbages. Three parental inbred lines MP01-11152，GB9266-18136, DT409-05121 were cultured in axillary bud’s leaves. A study on maximum plants regeneration was done in MS medium complementing to the material of MP01-11152, with MS as media by adding 6-BA with the concentration of 0, 2, 3, 4, 5 mg/L, then adding NAA with the concentration of 0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5 mg/L to screen the optimal concentration on this basis, Finally, the obtained optimal concentration of 2 mg/L 6-BA+0.4 mg/L NAA was used to research the plant regeneration on the material of MP01-11152、GB9266-18136、DT409-05121. The differentiation of shoot regeneration of MP01-11152 was 92% with 6-BA 2 mg/L and 122% with 2 mg/L 6-BA+0.4 mg/L NAA. GB9266-18136, one of the three parental inbred lines, obtained a high differentiation rate of 211%, MP01-11152’s differentiation 122%. DT409-05121’s differentiation is 82%. Medium for root was MS containing 0.3 mg/L NAA and could be cultured and developed into plantlets, the rooting rate as high as 98%. For the leaf in growth period of the axillary buds on spring cabbages, it could improve the differentiation of shoot in MS medium complemented with 6-BA or NAA. The genotypes have certain effects on shoot differentiation rate.