Difference between capacitation and frozen-thawed cell actin expression in boar sperm | 获能和冻融猪精子间细胞肌动蛋白表达的差异
2009
Han Mingming, Yanbian University, Longjing (China), College of Agriculture | Jin Yi, Yanbian University, Longjing (China), College of Agriculture | Fang Nanzhu, Yanbian University, Longjing (China), College of Agriculture
Chino. 分析获能与冻融猪精子细胞肌动蛋白表达的差异。提取获能和冻融的猪精子蛋白，经过SDS-PAGE分离后，进行Western-blot免疫印迹分析，检测获能与冻融猪精子间细胞肌动蛋白的区别。新鲜和冻融猪精子细胞肌动蛋白单体的分子质量均约为43 ku，但获能猪精子细胞肌动蛋白单体的分子质量约为43和86 ku。冻融的猪精子细胞肌动蛋白单体分子质量约为43 ku，但数量有所下降；获能后的猪精子出现了一种新型肌动蛋白亚单位，其分子质量约为86 ku。
Mostrar más [+] Menos [-]Inglés. The study was done in order to analyze the difference between capacitation and frozen-thawed boar sperm cells actin expression. After SDS-PAGE separation of the capacitation and frozen-thawed boar sperm, western blotting analysis was carried out to detect the distinction between capacitation and frozen-thawed boar sperm cell actin. Monomer molecular weight of fresh and frozen-thawed boar sperm cells actin was about 43 ku, but capacitation boar sperm cells actin was about 43 ku and 86 ku. Monomer molecular weight of frozen-thawed boar sperm cells actin was still 43 ku, but there was a decrease in number; a new actin subunit appeared in capacitation boar sperm, and the molecular weight was 86 ku .
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