In vitro effect of alpha-tocopherol and ascorbic acid supplementation on immunological indicators in bovine leukocytes following transportation
2009
Urban-Chmiel, R.,University of Life Sciences, Lublin (Poland). Inst. of Biological Bases of Animal Diseases | Wernicki, A.,University of Life Sciences, Lublin (Poland). Inst. of Biological Bases of Animal Diseases | Puchalski, A.,University of Life Sciences, Lublin (Poland). Inst. of Biological Bases of Animal Diseases | Dec, M.,University of Life Sciences, Lublin (Poland). Inst. of Biological Bases of Animal Diseases
The aim of the present study was to evaluate the effect of two different antioxidants, alpha-tocopherol and ascorbic acid, on the chemotactic activity of white blood cells and their susceptibility to Mannheimia haemolytica leukotoxin (Lkt) in vitro. The white blood cells were obtained from calves exposed to transport. Both antioxidants used increased the chemotactic activity of the leukocytes. Alpha-tocopherol supplementation at a concentration of 0.2 mg/mL and 0.5 mg/mL induced a significant increase. The greatest effect of ascorbic acid was observed at its concentration of 0.5 mg/mL. The results obtained in microtitration (MTT) assay also suggest that high concentrations of alpha-tocopherol (0.5 mg/mL) play a role in protecting bovine leukocytes against the cytotoxic effect of Lkt. The susceptibility of the leukocytes on M. haemolytica Lkt was lower in comparison with control about 6%. The antioxidants used in this study had a protective effect on cells participating in the immune response to M. haemolytica.
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