The morphological description and DNA tools analysis: for detection of duplications in the Czech germplasm collection of pepper (Capsicum annuum L.)
2010
Stavelikova, H.,Vyzkumny Ustav Rostlinne Vyroby, Olomouc (Czech Republic) | Hanacek, P.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Biologie Rostlin | Vyhnanek, T.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Biologie Rostlin
The Crop Research Institute in Olomouc, Czech Republic, holds a collection of pepper genetic resources. The collection consists of 504 accessions. The objective of this research was to find duplicates within the collection. Forty-one accessions were analyzed and divided into ten groups according as follows: 1. Astrachanskij, 2. Aufrechte Cayenne, 3. Bogyisloi, 4. Hatvani, 5. Japan Hontakka, 6. Japan Madarszen, 7. Kalocsai Fuszer (Edes), 8. Konservnyj Belyj 289, 9. Tetenyi and 10. Vinedale. Two approaches were used to detect the duplicates, morphological description and DNA polymorphism. Fifty-four morphological traits were evaluated: 1 character in seedlings, 8 characters in the plants, 10 characters in leaves, 10 characters in flowers and 25 characters in fruits. DNA polymorphism was analysed using the Simple Sequence Repeats method with 8 markers (Hpms 1-1, Hpms 1-5, Hpms 1-168, Hpms 1-172, Hpms 1-274, Hpms 2-21, Cams 163 and Cams 647) which are localised on different chromosomes. The results from DNA analysis were complemented with the morphological characterization. The duplicates were found in 4 groups as follows: 1. Astrachanskij, 4. Hatvani, 5. Japan Hontakka and 7. Kalocsai Fuszer (Edes).
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