New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss
2010
Dykova, I.,Akademie Ved, Ceske Budejovice (Czech Republic). Parazitologicky Ustav | Kostka, M.,Akademie Ved, Ceske Budejovice (Czech Republic). Parazitologicky Ustav | Wortberg, F.,Chemisches und Veterinaeruntersuchungsamt Stuttgart, Fellbach (Germany) | Nardy, E.,Chemisches und Veterinaeruntersuchungsamt Stuttgart, Fellbach (Germany) | Peckova, H.,Akademie Ved, Ceske Budejovice (Czech Republic). Parazitologicky Ustav
We studied amoebae associated with nodular gill disease (NGD) outbreaks in rainbow trout Oncorhynchus mykiss in fish farms in South-Western Germany. Gills of 12 diseased rainbow trout were examined in fresh, by isolation attempts, histologically and using in situ hybridisation (ISH). A total of nine amoeba strains of the genera Acanthamoeba (1), Hartmannella (2), Naegleria (1), Protacanthamoeba (1) and Vannella (4) were isolated and determined using light microscopical, ultrastructural and molecular methods. Specific molecular probes designed from the SSU rDNA sequences of individual amoeba strains were used for non-radioactive ISH in histological sections. Association of Naegleria sp. with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperplastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease.
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