Effect of pectic-oligosaccharides from orange albedo on enteropathogen bacteria adhesion-invasion of human gut epithelial cells in vitro | Efecto de pectic-oligosacáridos del albedo de la naranja sobre la adhesión e invasión de bacterias patógenas sobre células intestinales humanas in vitro
2009
Solanas, E., Instituto Aragonés de Ciencias de la Salud, Zaragoza (España). Lab. de Investigación Molecular | Hotchkiss, A.T. | Rastall, R.A.
The aim of the study was to investigate the ability of pectic oligosaccharides (POS) to inhibit adhesion and invasion of three relevant enteropathogen bacteria strains: the enterohemorrhagic E. coli 0157:H7, the enteropathogenic E. coli 0125:H6 and S. typhiumurium to human intestinal epithelial cell cultures using the human Caco-2 cell line. Two different POS fractions resulting in pectin extraction from orange albedo, were tested, POS1 (from filtrate) and POS 2 (from floc). Bacteria tested showed a higher adhesion with POS1 and POS2 at a dose of 2.5 mg/ml (P<0.05 for E. coli strains, P>0.05 for S. typhimurium), nevertheless invasion was significantly reduced (P<0.001) for the three bacteria strains, especially for E. coli 125:H6 when they were incubated with either POS1 or POS2 in DPBS on Caco-2 monolayers. Reduction of zeta potential of bacteria strains on POS solutions could produce an apparent or unspecific attachment of bacteria to cells which did not imply a real bacteria attachment to cells and consequent cell invasion.
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