Phenolocs and phenol oxidizing enzymes activity as index for screening ber (Ziziphus mauritiana) germplasm against powdery midew (Oidium erysiphoides)
2009
Nallathambi, P. | Umamaheswari, C | More, T.A.
The total phenol content and activity of phenol oxidizing enzyme, viz peroxidase (PO) and polyphenol oxidase PPO) in health and powdery mildew colonized leaves and fruits of ber at different groeth stages is reported in this paper. In response to partial colonozation of the test pathogen Oidium erysiphoides f.sp. zizphi in immature fruits of the resistant genotypes viz Banarasi Karaka, Saferchandi and Illaichi high level of total phenplics and activity of PO and PPO were recorded. In highly susceptible cv. Gola and Umran the infection could elicit lesser response. In general immature and mature fruits contain less quantity of phenolics as compared to different growth stage of leaves. The wild species (Z. nummularia) accumulated significantly high (2.98 mg/g) phenolics, followed by Saferchandi (1.949 mg/g) and Illachi (1.901 mg/g). Similar was the case in elaboration of PO (327.29 units/min/g) and PPO (32.58 units/mg of protein) activity in this particular genotype. In order to screen large number of ber genotypes in short span of time, random sampling of the immature leaves and fruits are appropriate for analyzing these biochemical molecules as demarcating indicate of ber powdery mildew resistance.
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