Effect Of Thawing Regime On Post-Thaw Quality Of Ram Semen Frozen In Tris, Citrate And Phosphate Based Diluents
2009
Nema, S.P. | Dhami, A.J. | Kavani, F.S.
To study the optimum thawing protocol for freezing of ram semen, a factorial experiment was carried out using 3 buffers (viz, tris fructose citric acid egg yolk glycerol - TFCED, phosphate buffer glucose fructose sodium citrate potassium chloride egg yolk glycerol - a synthetic phosphate medium - phos, and sodium citrate glucose egg yolk glycerol - SCGEG), 3 thawing temperatures (37°, 40° and 50°C) and 2 thawing periods (8 and 15 sec) for freezing and thawing of semen of 5 Patanwadi rams. Irrespective of diluents used, the optimum - thawing regime was 40°C temperature for 8 seconds in terms of post thaw motility and acrosome score for French medium straws. Among the 3 diluents used, TFCEG appeared the best for cryopreservation of ram semen.
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