The Immunological Responses to Various Cell Wall Fractions of Pasteurella multocida in Chicken
2003
Esmaily, F., Razi Vaccine and Serum Research Institute, Tehran, Iran | Jabari, A.R., Razi Vaccine and Serum Research Institute, Tehran, Iran | Sotoodehnia, A., Razi Vaccine and Serum Research Institute, Tehran, Iran | Moazeni Jula, G.R., Razi Vaccine and Serum Research Institute, Tehran, Iran
Various cell wall fractions of Pasteurella multocida vaccine strain serotype A:1 including sonicated antigen, heat stable (HS), lipopolysaccharide-protein complex (LPS-P), outermembrane protein (OMP), capsular (CAP) and potassium thiocyanate (KSCN)–extracted proteins were extracted. Their immunogenecity was studied and compared with inactivated whole cell vaccine in vitro and in vivo. The protein estimation and the polypeptide pattern of these antigens were also compared by Lowery and SDS-PAGE methods. In general the immunological responses to these fractions were varied. The chickens immunized with KSCN–extract antigen and OMP showed the highest protection when they were challenged with homologous strain, while HS and LPS antigens showed the lowest protection. These findings are in correspondence with the end–point titer of antibodies using ELISA. Cross-reaction was also observed by agar gel difussion test between the fractions and raised antisera in chickens especially with KSCN-extract, OMP and CAP antigens. The results suggest that both OMP and KSCNextract protein as more effective immunogens could be good candidates for development of a subunit vaccine substitute for current whole cell formalized vaccine against fowl cholera infection.
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