An In vivo Assay for Biological Activity of Synthesized Recombinant Human Growth Hormone
2003
Tafreshi, A.P., The National Research Center for Genetic Engineering and Biotechnology of Iran, Tehran, Iran | Sanati, M.H., The National Research Center for Genetic Engineering and Biotechnology of Iran, Tehran, Iran
The biological activity of the recombinant growth hormone (GH) synthesized in The National Research Center for Genetic Engineering and Biotechnology (NRCGEB) was evaluated in vivo. In this regard a hypophysectomized rat model was required. Using a para pharyngeal approach, the sphenoid bone of rats was drilled and the hypophysis was out successfully by aspiration. Following a 7-day recovery period, rats were injected subcutaneously either with the recombinant human GH or the Novo, as standard. The results show that both hormones have an equal biological activity and resulted in a 100% growth increment equal to that in untreated intact rats indicating that the recombinant GH can be introduced as a good replacement of endogenous GH.
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