Identification of Microsatellite Markers in Sheep (Bulochi Sheep)
2008
Esmaeil Khaniyan, Saeid | Nejati, Ardeshir | Afraz, Fazl Ol-Lah | Sane`i, Mohammad Reza
Baluchi sheep are the most numerous sheep breed in Iran and breeding station is located in Abbas Abad, Mashad. In this study, genetic diversity within this population and also polymorphism in 73 microsatellite loci were calculated. Each of the loci was investigated using 145 DNA samples. PCR conditions for all loci were optimized and PCR products were run on polyacrylamide gels and bands revealed by a silver staining procedure. Based on our results, all loci, except BMS4000 and OarAE54, amplified and were polymorphic. MCM120 (0.9232) and TGLA387 (0.6839) have the highest and the lowest PIC values respectively. The most and the least number of True allele observed in MCM120 (21 alleles) and MCM494, MCM541L (9 alleles) respectively and the most and the least number of effective allele belong to CSRD144 (14.9664) and TGLA387 (3.6255) respectively. Average heterozygosity over all loci in this population calculated as 0.8764. All loci, based on chi-square and likelihood ratio tests, were not in Hardy-Winberg equilibrium (HWE) and only MCM120 with G2 was in HWE. High genetic diversity in Baluchi sheep population, based on studied loci, can be concluded from the result of present investigation.
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