Pathogenicity of Verticillium lecanii (=Lecanicillium muscarium.) on brown tail moth Euproctis chrysorrhoea (L.) larvae. at laboratory condition
2003
M. J., Farsi, Ph. D. student of agricultural entomology | H., Askary, Res. Assist. Prof. of research institute of forests and ranges | Kh., Talebi, Assoc. Prof. and Assoc. Prof. Of college of agriculture, Tehran University | A., Kharrazi Pakdel, Assoc. Prof. and Assoc. Prof. Of college of agriculture, Tehran University
Brown tail moth,Euproctis chrysorrhoea is an important defoliator of oak and a number of hardwood tree species. Its irritant hairs make it a sanitary important insect on humans. Introduction of a suitable biological agent with the other control measures is quite important especially in the case of population outbreaks. V. lecanii strain DAOM 198499 grown on a solid culture medium was used for pathogenicity and bioassay procedures. Suspensions of 104, 105, 106, 107 and 108 conidia/ml with a control (distilled water) containing Triton X-100 were used on third instar larvae in a complete randomized design with three replications. In other experiments, treatments included two suspensions (106 and 108 conidia/ml) and two durations of 97±2 percent RH supply (24 and 48 hours after spore application), with or without desiccant material on third instar larvae. A factorial complete randomized design was applied.In the first experiment suspensions of 107 and 108 conidia/ml gave a significantly better result than the others (p0.01). LC50, determined by proc probit analysis required a concentration of 7.2×106 conidia/ml. Over 50% larval mortality was observed only in 107 and 108 conidia/ml suspensions, and LT50 was measured by life test procedure as 7 ± 0.31 and 5 ± 0.22 days, respectively. Maintenance for 48 hours at high RH gave an average mortality rate of 90.95%, significantly different from the 40.44% mortality rate after 24 hours (p0.01). This is the first report of the pathogenicity of this strain of Verticillium lecanii on brown tail moth larvae. Key words: Verticillium lecanii,Lecanicillium muscarium, Euproctis chrysorrhoea, biological control, bioassay
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