Study of ammonia excretion value by rainbow trout (Oncorhynchus mykiss) eggs from fertilization until hatch stage
2009
Rezaei Tavabe, K., Research Expert of Tehran University | Mirvaghefi, A.R., Tehran University, Natural Resources Faculty | Rafiee, Gh.R., Tehran University, Natural Resources Faculty | Mojazi Amiri, B., Tehran University, Natural Resources Faculty | Abdolhai, H., Iranian Fisheries Research Institute | Taheriazad, H. L., Natural Resources Faculty of Tarbiat Modarres University
The main objective of the present study was to determine ammonia excretion by rainbow trout eggs at different stages of incubation period. The research was carried out at Namroud rainbow trout hatchery center in winter 2005. For this purpose, two incubators with same condition as experiment unit were selected which one of them as experimental incubator with approximately 60000 eggs and another one without any eggs. Water and egg sampling during incubation period was conducted weekly. Synchronously with the egg and water sampling, water physico-chemical factors which affect to ammonia-unionized ammonia balance such as pH, Temperatures and soluble materials by digital sensitive apparatus were measured. Afterwards ammonia in water samples were measured by filter-photometry Palin test apparatus and total nitrogen in sampled eggs were determined by Kejletic method. Ammonia excretion value during incubation period stages from fertilization until hatch was 0.0012±0.0003, 0.0019±0.0005, 0.0088±0.0009, 0.0089±0.0013 and 0.0082±0.0011 ppm respectively. The results showed with ammonia excretion increasing during incubation period total nitrogen at egg texture with decreasing trend had significant differences (P>0.05) during this period that indicates ammonia excretion is resulted from organic component of yolk metabolism activities. Keywords: Rainbow trout; Namroud; Incubator; Embryonic; Ammonia
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