Preparation of Semi-purified Laminated Layer of Hydatid Cyst for Diagnosis of Infection
2001
Taherkhani, H., Hamadan University of Medical Sciences, Hamadan, Iran | Rogan, M.T., University of Salford, Salford M5 4WT, UK.
A semi-purified laminated layer (LL) fraction of cyst wall of Echinococcus granulosus was prepared by affinity chromatography. Also its antigenic reactivity evaluated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE analysis of the LL fractions showed that the bands in the 50-55kDa region and the 25-31kDa region were greatly reduced in semi-purified fraction in comparison with unpurified sample, which presumably reflected to removal of heavy and light immunoglobulin chains. An immunoblot data also confirms considerable reduction in the presence of IgG chains within the semipurified fraction. The current study indicated that the bands at 66, 55 and 27kDa were significant molecules for diagnosis of human cystic hydatidosis patients after purification of the crude sample.
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