Investigation Of Optimum Time To Yield The Optimum Titre In Cultivation Of Measles Virus Vaccine Alk-C Strain In Monolayer Culture Of Chicken Fibroblast Cells. ccine.
1999
Nasiri , Saber
It,s necessary to do a deep regearch to detennine optimum time to yield the best harvest of Measele virns vaccine. Intentionaly Monalayer chick embrio fibroblast cell provided for and a detetminared quantity of vilUS inoculated to. As virns is as absolute parasit absorbed by receplors and little by little inter to the cell and being increased. Harvesting time usually begins about 72-96 hours after inoculation or after appearane of cyto pathic effect (CPE), and goes on per 24 hours on the condition that cells resist and do not detach from the fluid of surface of the bottle. Harvested series titred separatly. Titve increasing in a particular time confinn the optimum in harvesting, that is very important in production of MEASL + ES vaccine
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