In vitro micropropagation of Philodendron cannifolium
2008
Han, B.H. (National Horticultural Research Institute, RDA, Suwon, Republic of Korea), E-mail: [email protected] | Park, B.M. (Chonbuk National University, Iksan, Republic of Korea)
In order to micropropagate uniform plantlets of Philodendron cannifolium in vitro, the shoot tips were cultured on MS media supplemented with 0.5~10.0 mg/L BA or 0.05~0.1 mg/L thidiazuron (TDZ). The adventitious multi-bud clusters from basal part of shoots were formed on MS media containing 2.0~5.0 mg/L BA or 0.05~0.1 mg/L TDZ. But the shoots grown on MS media with TDZ showed necrosis by the lack of chlorophyll. The adventitious multi-bus clusters were cut into 5~7 mm sections and cultured on MS media containing BA and TDZ for shoot proliferation. Shoots were proliferated vigorously on MS medium supplemented with 1.0~3.0 mg/L BA with up to 30 shoots. But abnormally swollen hard calli were formed from basal parts of shoots on MS media with TDZ and high concentration of BA (10.0 mg/L). The proliferated shoots on same media also showed necrosis by the lack of chlorophyll. The shoot growth and rooting were favorable on MS media containing 0.5~2.0 mg/L IBA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1:vermiculite 1 or vermiculite alone. Fifteen mL of liquid medium containing 10 g/L activated charcoal and 30 g/L sucrose were added in same vessels after small shoots were proliferated to stimulate shoot growth and rooting. After 8 weeks in culture, the shoots were dipped into high concentration of IBA solution. and planted in soil mexed with perlite 1 : vermiculite 1. The shoot growth and rooting were favorable in dipping treatments of 500~2,000 ppm IBA solutions for 10 sec.
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