Highly sensitive detection method of verotoxins produced by enterohaemorrhagic Escherichia coli O-157:H7 using a protein chip
2010
Tanabe, R., Chubu Univ., Kasugai, Aichi (Japan) | Cha, B.Y. | Yonezawa, T. | Woo, J.T. | Nagai, K.
Enterohaemorrhagic Escherichia coli O-157:H7 produces verotoxins called Shiga toxin or Shiga-like toxin. The type 1 and type 2 toxins present different immunological and physicochemical characteristics, and cause symptoms such as watery diarrhea, bloody diarrhea, severe stomach ache, fever and vomiting. The toxins can be assayed by several methods, including ELISA and PCR, with some limitations as to sensitivity. In the present study, we used a ProteoChip, which requires smaller volumes of reagents and allows detection of lower concentrations of the toxins, compared with the conventional assay.
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