Molecular Analysis of Korean Isolate of Barley mild mosaic virus (Iks Isolate)
2009
Choi, M.K. (Jeollabuk-Do Agricultural Research and Extension Services, Iksan, Republic of Korea) | Seralathan, Kamala-Kannan (Chonbuk National University, Iksan, Republic of Korea) | Oh, B.T. (Chonbuk National University, Iksan, Republic of Korea) | Park, J.C. (Honam Agricultural Research Institute, NICS, RDA, Iksan, Republic of Korea) | Lee, G.W. (Chonbuk National University, Iksan, Republic of Korea) | Lee, K.J. (Chonbuk National University, Iksan, Republic of Korea) | Park, Y.J. (Chonbuk National University, Iksan, Republic of Korea), E-mail: [email protected]
The complete nucleotide sequences of both RNA of an isolated Barley mild mosaic virus (BaMMV) from Iksan, Korea, have been determined. RNA1 was 7273 nucleotides long and encodes for a polyprotein of 2261 amino acids, which contains the eight putative functional proteins. RNA2 was 3520 nucleotides long and encodes for a polyprotein of 894 amino acids, which contains two functional proteins. Results of multiple sequence alignment showed 92.9% similarity with Na1 isolate, followed by Sil, UK(F), Asl1, Remis M and UK(M) isolates, respectively. Comparison of the BaMMV-Iks polyproteins with the corresponding proteins of BaMMV-Na1 isolates showed 95% amino acid sequence identity. The phylogenetic analysis revealed that Iks isolate was closely related to Na1 strain and have a common origin.
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