Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae
2010
Hirasaki, M.., Osaka Univ., Suita (Japan) | Nakamura, F. | Yamagishi, K. | Numamoto, M. | Shimada, Y. | Uehashi, K. | Muta, S. | Sugiyama, M. | Kaneko, Y. | Kuhara, S. | Harashima, S.
Expression profiles of protein phosphatase (PPase) disruptants were analyzed by use of Pearson's correlation coefficient to find profiles that correlated with those of 316 Reference Gene (RG) disruptants harboring deletions in genes with known functions. Twenty-six deltappase disruptants exhibited either a positive or negative correlation with 94 RG disruptants when the p value for Pearson's correlation coefficient was 0.2. Some of the predictions that arose from this analysis were tested experimentally and several new deltappase phenotypes were found. Notably,deltasit4 and deltasiw14 disruptants exhibited hygromycin B sensitivity, deltasit4 and deltaptc1 disruptants grew slowly on glycerol medium, the deltaptc1 disruptant was found to be sensitive to calcofluor white and congo red, while the deltappg1 disruptant was found to be sensitive to congo red. Because on-going analysis of expression profiles of Saccharomyces cerevisiae disruptants is rapidly generating new data, we suggest that the approach used in the present study to explore PPase function is also applicable to other genes.
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