A simple method for long-term cryopreservation of Calonectria ilicicola on barley grains
2010
Ochi, S., National Agricultural Research Center, Tsukuba, Ibaraki (Japan) | Nakagawa, A.
We developed a simple method for long-term preservation of the soybean red crown rot fungus, Calonectria ilicicola, using barley grains. Autoclaved barley grains were inoculated with the fungus, then incubated at 25degC for 1 month. After incubation, grains were dried to approximately 3% moisture content, and stored at 4, -20, or -80degC for 3 years. C. ilicicola preserved on barley grains at -80degC remained viable without any change in mycelial growth and virulence. These results showed that C. ilicicola can be successfully cryopreserved for extended periods on barley grains at -80degC. We also confirmed that cultures preserved on barley grains are suitable for direct use without further manipulation as inocula in pathogenicity tests.
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