Characterization of the genomes of Aujeszky's disease virus isolated in Korea
2009
Hyun, B.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, I.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Pyo, H.M., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Cha, S.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Park, J.Y., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Song, J.Y., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Cho, I.S., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Yang, C.B., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | An, S.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Lee, J.B., Konkuk University, Seoul, Republic of Korea
The molecular genetic characterization of Aujeszky's disease virus (ADV) Yangsan strain (ADV-YS), a Korean isolate, was investigated by analyzing the electrophoresis patterns and the physical maps of the viral DNA digested with various endonucleases. To establish DNA library for ADV-YS, twelve major BamHI restricted segments were cloned. Each location of the segments in the ADV genome was determined by sequence comparison with the sequences reported in Genbank and those sequences of the both termini of the segments. Physical maps were constructed based on the electrophoresis patterns of the digested viral DNA by restriction endonuclease and the results of Southern blot analyses with various DIG labeled probes originated from those of enzyme restricted segments of virulent (Shope) and avirulent (Bartha) strain. Comparing ADV-YS with a standard strainof Kaplan in the maps of restriction enzymes, following major respects were identified: (ⅰ) disappearance of BamHI restriction site between the first and second BamHI segments, (ⅱ) creation of the BamHI restriction site in the fifth segment, and (ⅲ) generation of the BglⅡ site in the unique short (Us) regions. The genome of ADV-YS also contains a type 2 herpesvirus DNA molecule (in which the Us region only inverts itself relative to the unique longregion) like all other ADV strains except Norden strain(type3), analyzed up to date. The size of the ADV genome estimated from the sizes of the restriction enzyme fragments, was approximately 145.3kb (BamHI) or 145.3kb (BglⅡ) 145.4kb (BglⅡ). BamHI enzyme cleavage pattern were compared among the five Korean ADV isolates: Yangsan, Yongin, Dangjin, Jincheon and Iksan strains. Difference either in the number or in the size of the DNA fragments, suspected regions of termini of IR and TR, could be detected among all five strains.
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