Leptin gene polymorphism in cattle bred in Lithuania
2010
Krasnopiorova, N., Lithuanian Veterinary Academy, Kaunas (Lithuania) | Baltrenaite, L., Lithuanian Veterinary Academy, Kaunas (Lithuania) | Navakauskaite, G., Lithuanian Veterinary Academy, Kaunas (Lithuania) | Morkuniene, K., Lithuanian Veterinary Academy, Kaunas (Lithuania) | Miceikiene, I., Lithuanian Veterinary Academy, Kaunas (Lithuania)
The aim of this study was to investigate leptin gene polymorphism and distribution of different alleles and genotypes in cattle bred in Lithuania. Samples to investigate leptin gene polymorphism were taken from 61 unrelated individual belonging to 6 breeds: Lithuanian Black and White (12), Lithuanian Red (14), Limousin (8), Simental (7), Charolaise (8), Holstein (12). DNA was extracted from hair roots. Leptin gene polymorphysm was investigated by PCR-RFLP method (Choudhary et al., 2005). Summarizing, we found that 522 bp leptin gene fragment is polymorphic in all the cattle breeds studied. Mutant A allele was found with lower frequency than G allele. Holstein population showed the highest GG homozygote genotype. The further research is planned to search for cattle leptin gene polymorphism found with production traits.
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