Efficient micropropagation of chickpea (Cicer arietinum L.)
2009
Rekha, K.T. | Thiruvengadam, M., National Chung-Hsing Univ., 250 Kuo-Kuang Road, Taichung-402, Taiwan (Republic of China). Graduate Inst. of Biotechnology
A rapid and reproducible protocol for in vitro shoot induction and plantlet regeneration was achieved from the cotylendonary node and axillary buds of chickpea (Cicer arietinum L.). Multiple shoots were induced from cotyledonary node and axillary buds grown on Murashige and Skoog (MS) medium (Murashige and Skoog 1962) with B sub5 (Gamborg et al 1968) vitamins (MSB sub5) containing 6-benzyl aminopurine (BAP), Kinetin (KIN) and adeninedisulphate (Ads) individually. The maximum number of multiple shoots and the longest shoots were obtained from the MS medium containing 1.0 mg/L BAP in cotyledonary node and 1.5 mg/L BAP in axillary buds. Of the two different explants tested, cotyledonary nodes produced more number of shoots. Elongated shoots were harvested and subsequently transferred to MS medium containing 0.5-2.0 mg/L of either indole-3 butyric acid (IBA), indole-3-acetic acid (IAA) or napthalene acetic acid (NAA) for root induction. Maximum rooting response was observed in the MS medium containing 1.5 mg/L IBA. In vitro regenerated plantlets that were transferred to the soil medium attained 99% survival rate; the plants also looked healthy with no visually detectable phenotypic variations. This protocol yielded on average of 15 plants per cotyledonary node explant in 25-d culture.
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