Quantitative and qualitative determination of enrofloxacin residues in fish tissue
2009
Djordjevic, V., Institute of Meat Hygiene and Technology, Belgrade (Serbia) | Baltic, M., University of Belgrade, Belgrade (Serbia). Faculty of Veterinary Medicine | Cirkovic, M., University of Novi Sad, Novi Sad (Serbia). Faculty of Agriculture | Kilibarda, N., Veterinary Specialistic Institute, Subotica (Serbia) | Glamoclija, N., University of Belgrade, Belgrade (Serbia). Faculty of Veterinary Medicine | Stefanovic, S., Institute of Meat Hygiene and Technology, Belgrade (Serbia) | Miscevic, M., Serbian Chamber of Commerce, Belgrade (Serbia)
The aim of this paper was to investigate enrofloxacin residues in carp tissues (liver, kidneys, muscle tissue) after a five-days oral application of the drug in therapeutic doses. Two analytical methods were applied for the purpose of investigation, microbiological (screening) method and HPLC method with fluorescence detection. After the 5-day oral treatment of carps, enrofloxacin residues in tissues were determined up to the 10th day after the end of the drug application. Enrofloxacin content determined by the HPLC method was lower than MRL. Drug residues were determined in liver on the 6th day after treatment, in kidney on the 7th day and in muscle on the 9th day after treatment. The results of enrofloxacin residues determination by screening method on the medium with E. coli ATCC 11303, pH 8 show that this procedure can be used for qualitative determination of enrofloxacin. Screening method allows determination of enrofloxacin in fish tissues below the MRL.
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