Axenically culturing the bryophytes: a case study of the moss Dicranum scoparium Hedw. (Dicranaceae, Bryophyta)
2009
Vujicic, M., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany and Botanical Garden Jevremovac | Sabovljevic, A., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany and Botanical Garden Jevremovac | Sabovljevic, M., University of Belgrade, Belgrade (Serbia). Faculty of Biology, Institute of Botany and Botanical Garden Jevremovac
The aim of this study was to establish exenic culture of the moss Dicranum scoparium a counterpart of rare and widly endangered D. viride. The media contents, as well as light and temparature were varied to find the optimal conditions for spore germination, protonema growing, bud formation and gametophyte development. The best conditions for micropropagation or axenically bryo-farming is to grow D. scoparium on the MS medium enriched with sucrose (1.5%), at 18-20 deg C independent of light length condition.
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