Characterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae
2009
Loncar, N., University of Belgrade, Belgrade (Serbia). Faculty of Chemistry | Bozic, N., University of Belgrade, Belgrade (Serbia). Institute of Chemistry, Technology and Metallurgy, Department of Chemistry | Nenadovic, V., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Insect Physiology and Biochemistry | Ivanovic, J., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Insect Physiology and Biochemistry | Vujcic, Z., University of Belgrade, Belgrade (Serbia). Faculty of Chemistry
The pH along the midgut of Morimus funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.
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