Etiological and molecular biological investigation of carpine herpesvirus 1 isolated in Bulgaria
2009
Peshev, R. | Sirakov, I. | Nedelchev, N. | Karadzhov, S., National Diagnostic and Research Veterinary Medical Institute, Sofia (Bulgaria)
In this paper is described the isolation and identification of CHV 1 causing goat infection and by using the physicochemical and molecular biological methods to study some biological peculiarity of virus. Five CHV 1 strains are isolated by cell cultures and identified as goat herpesvirus es from different Bulgarian regions. After electron microscopy the viral agents with typical size and morphology for herpesvirus are established. The conclusions in this paper are the following: 1) Isolation and adaptation of five goat herpesviruses were possible only after treatment with DMSO; 2) CHV 1 viral strains can be successfully adapted on primary and permanent cell culture from bovine origin; 3) Isolated viral agents have cultural, biochemical and morphological characteristics typical for CHV 1; 4) The amplicons of all virus strain after performed PCR assay have the same size and mobility as the reference CHV 1 strains; 5) Statistically significant increasing of MVNT antibody titers in recovalescent sera is an evidence for the circulation of CHV 1 in goat farm.
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